Delivery of neurturin by AAV2 (CERE‐120)‐mediated gene transfer provides structural and functional neuroprotection and neurorestoration in MPTP‐treated monkeys
Identifieur interne : 001326 ( Main/Exploration ); précédent : 001325; suivant : 001327Delivery of neurturin by AAV2 (CERE‐120)‐mediated gene transfer provides structural and functional neuroprotection and neurorestoration in MPTP‐treated monkeys
Auteurs : Jeffrey H. Kordower [États-Unis] ; Christopher D. Herzog [États-Unis] ; Biplob Dass [États-Unis] ; Roy A. E. Bakay [États-Unis] ; James Stansell Iii [États-Unis] ; Mehdi Gasmi [États-Unis] ; Raymond T. Bartus [États-Unis]Source :
- Annals of Neurology [ 0364-5134 ] ; 2006-12.
Abstract
Objective: We tested the hypothesis that gene delivery of the trophic factor neurturin could preserve motor function and protect nigrostriatal circuitry in hemiparkinsonian monkeys. Methods: An adeno‐associated virus–based vector encoding human neurturin (AAV2‐NTN; also called CERE‐120) was injected into the striatum and substantia nigra of monkeys 4 days after a unilateral intracarotid injection of N‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine (MPTP) rendered them hemiparkinsonian. Control hemiparkinsonian monkeys received either AAV2 encoding green fluorescent protein or formulation buffer. Results: Although stable deficits were seen in all control monkeys, AAV2‐NTN significantly improved MPTP‐induced motor impairments by 80 to 90% starting at approximately month 4 and lasting until the end of the experiment (month 10). AAV2‐NTN significantly preserved nigral neurons, significantly preserved striatal dopaminergic innervation, and activated phospho‐extracellular signal–regulated kinase, consistent with a mechanism involving a trophic factor–initiated molecular cascade. Histological analyses of numerous brain regions, including the cerebellum, showed normal cytoarchitecture and no aberrant pathology. Interpretation: These data demonstrate that AAV2‐NTN (CERE‐120) can preserve function and anatomy in degenerating nigrostriatal neurons and are supportive of ongoing clinical tests in Parkinson's disease patients. Ann Neurol 2006;60:706–715
Url:
DOI: 10.1002/ana.21032
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Delivery of neurturin by AAV2 (CERE‐120)‐mediated gene transfer provides structural and functional neuroprotection and neurorestoration in MPTP‐treated monkeys</title><author><name sortKey="Kordower, Jeffrey H" sort="Kordower, Jeffrey H" uniqKey="Kordower J" first="Jeffrey H." last="Kordower">Jeffrey H. Kordower</name></author><author><name sortKey="Herzog, Christopher D" sort="Herzog, Christopher D" uniqKey="Herzog C" first="Christopher D." last="Herzog">Christopher D. Herzog</name></author><author><name sortKey="Dass, Biplob" sort="Dass, Biplob" uniqKey="Dass B" first="Biplob" last="Dass">Biplob Dass</name></author><author><name sortKey="Bakay, Roy A E" sort="Bakay, Roy A E" uniqKey="Bakay R" first="Roy A. E." last="Bakay">Roy A. E. Bakay</name></author><author><name sortKey="Stansell Iii, James" sort="Stansell Iii, James" uniqKey="Stansell Iii J" first="James" last="Stansell Iii">James Stansell Iii</name></author><author><name sortKey="Gasmi, Mehdi" sort="Gasmi, Mehdi" uniqKey="Gasmi M" first="Mehdi" last="Gasmi">Mehdi Gasmi</name></author><author><name sortKey="Bartus, Raymond T" sort="Bartus, Raymond T" uniqKey="Bartus R" first="Raymond T." last="Bartus">Raymond T. Bartus</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:3F1A4BB0C057CD1E4158F953F9ABABE6B2D75716</idno><date when="2006" year="2006">2006</date><idno type="doi">10.1002/ana.21032</idno><idno type="url">https://api.istex.fr/document/3F1A4BB0C057CD1E4158F953F9ABABE6B2D75716/fulltext/pdf</idno><idno type="wicri:Area/Main/Corpus">002734</idno><idno type="wicri:Area/Main/Curation">002391</idno><idno type="wicri:Area/Main/Exploration">001326</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Delivery of neurturin by AAV2 (CERE‐120)‐mediated gene transfer provides structural and functional neuroprotection and neurorestoration in MPTP‐treated monkeys</title><author><name sortKey="Kordower, Jeffrey H" sort="Kordower, Jeffrey H" uniqKey="Kordower J" first="Jeffrey H." last="Kordower">Jeffrey H. Kordower</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Illinois</region></placeName><wicri:cityArea>Department of Neurological Sciences, Rush University Medical Center, Chicago</wicri:cityArea></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Illinois</region></placeName><wicri:cityArea>Department of Neurosurgery, Rush University Medical Center, Chicago</wicri:cityArea></affiliation></author><author><name sortKey="Herzog, Christopher D" sort="Herzog, Christopher D" uniqKey="Herzog C" first="Christopher D." last="Herzog">Christopher D. Herzog</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Ceregene, San Diego</wicri:cityArea></affiliation></author><author><name sortKey="Dass, Biplob" sort="Dass, Biplob" uniqKey="Dass B" first="Biplob" last="Dass">Biplob Dass</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Illinois</region></placeName><wicri:cityArea>Department of Neurological Sciences, Rush University Medical Center, Chicago</wicri:cityArea></affiliation></author><author><name sortKey="Bakay, Roy A E" sort="Bakay, Roy A E" uniqKey="Bakay R" first="Roy A. E." last="Bakay">Roy A. E. Bakay</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Illinois</region></placeName><wicri:cityArea>Department of Neurosurgery, Rush University Medical Center, Chicago</wicri:cityArea></affiliation></author><author><name sortKey="Stansell Iii, James" sort="Stansell Iii, James" uniqKey="Stansell Iii J" first="James" last="Stansell Iii">James Stansell Iii</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Illinois</region></placeName><wicri:cityArea>Department of Neurological Sciences, Rush University Medical Center, Chicago</wicri:cityArea></affiliation></author><author><name sortKey="Gasmi, Mehdi" sort="Gasmi, Mehdi" uniqKey="Gasmi M" first="Mehdi" last="Gasmi">Mehdi Gasmi</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Ceregene, San Diego</wicri:cityArea></affiliation></author><author><name sortKey="Bartus, Raymond T" sort="Bartus, Raymond T" uniqKey="Bartus R" first="Raymond T." last="Bartus">Raymond T. Bartus</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Ceregene, San Diego</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Annals of Neurology</title><title level="j" type="sub">Official Journal of the American Neurological Association and the Child Neurology Society</title><title level="j" type="abbrev">Ann Neurol.</title><idno type="ISSN">0364-5134</idno><idno type="eISSN">1531-8249</idno><imprint><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2006-12">2006-12</date><biblScope unit="volume">60</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="706">706</biblScope><biblScope unit="page" to="715">715</biblScope></imprint><idno type="ISSN">0364-5134</idno></series><idno type="istex">3F1A4BB0C057CD1E4158F953F9ABABE6B2D75716</idno><idno type="DOI">10.1002/ana.21032</idno><idno type="ArticleID">ANA21032</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0364-5134</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Objective: We tested the hypothesis that gene delivery of the trophic factor neurturin could preserve motor function and protect nigrostriatal circuitry in hemiparkinsonian monkeys. Methods: An adeno‐associated virus–based vector encoding human neurturin (AAV2‐NTN; also called CERE‐120) was injected into the striatum and substantia nigra of monkeys 4 days after a unilateral intracarotid injection of N‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine (MPTP) rendered them hemiparkinsonian. Control hemiparkinsonian monkeys received either AAV2 encoding green fluorescent protein or formulation buffer. Results: Although stable deficits were seen in all control monkeys, AAV2‐NTN significantly improved MPTP‐induced motor impairments by 80 to 90% starting at approximately month 4 and lasting until the end of the experiment (month 10). AAV2‐NTN significantly preserved nigral neurons, significantly preserved striatal dopaminergic innervation, and activated phospho‐extracellular signal–regulated kinase, consistent with a mechanism involving a trophic factor–initiated molecular cascade. Histological analyses of numerous brain regions, including the cerebellum, showed normal cytoarchitecture and no aberrant pathology. Interpretation: These data demonstrate that AAV2‐NTN (CERE‐120) can preserve function and anatomy in degenerating nigrostriatal neurons and are supportive of ongoing clinical tests in Parkinson's disease patients. Ann Neurol 2006;60:706–715</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Californie</li><li>Illinois</li></region></list><tree><country name="États-Unis"><region name="Illinois"><name sortKey="Kordower, Jeffrey H" sort="Kordower, Jeffrey H" uniqKey="Kordower J" first="Jeffrey H." last="Kordower">Jeffrey H. Kordower</name></region><name sortKey="Bakay, Roy A E" sort="Bakay, Roy A E" uniqKey="Bakay R" first="Roy A. E." last="Bakay">Roy A. E. Bakay</name><name sortKey="Bartus, Raymond T" sort="Bartus, Raymond T" uniqKey="Bartus R" first="Raymond T." last="Bartus">Raymond T. Bartus</name><name sortKey="Dass, Biplob" sort="Dass, Biplob" uniqKey="Dass B" first="Biplob" last="Dass">Biplob Dass</name><name sortKey="Gasmi, Mehdi" sort="Gasmi, Mehdi" uniqKey="Gasmi M" first="Mehdi" last="Gasmi">Mehdi Gasmi</name><name sortKey="Herzog, Christopher D" sort="Herzog, Christopher D" uniqKey="Herzog C" first="Christopher D." last="Herzog">Christopher D. Herzog</name><name sortKey="Kordower, Jeffrey H" sort="Kordower, Jeffrey H" uniqKey="Kordower J" first="Jeffrey H." last="Kordower">Jeffrey H. Kordower</name><name sortKey="Stansell Iii, James" sort="Stansell Iii, James" uniqKey="Stansell Iii J" first="James" last="Stansell Iii">James Stansell Iii</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/ParkinsonV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001326 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001326 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= ParkinsonV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:3F1A4BB0C057CD1E4158F953F9ABABE6B2D75716
|texte= Delivery of neurturin by AAV2 (CERE‐120)‐mediated gene transfer provides structural and functional neuroprotection and neurorestoration in MPTP‐treated monkeys
}}
| This area was generated with Dilib version V0.6.23. |  |